Mirza, Shama
mirza@uwm.eduKenwood IRC 2076D, , , , , ,

Mirza, Shama

Associate Professor / Director of the Shimadzu Laboratory
Chemistry & Biochemistry

Mirza Group Website

Research Area

Our research is focused on making precision medicine a reality. We examine which proteins are key biomarkers for a disease condition. Our lab works hand-in-hand with clinicians to implement discoveries as soon as possible. Our primary focus is to carry out the comprehensive characterization of proteins using mass spectrometry in order to better understand protein functions and interactions under normal and disease states; primary focus being on glioblastoma, obstructive uropathy and endometrial cancer. The lab is also involved in developing novel technologies for mass spectrometry (MS)-based research.

New and efficient therapeutic targets for Glioblastoma

Glioblastoma (GBM) is the most common and very aggressive type of the primary brain tumors affecting about 17,000 Americans annually. Even after initial treatment, almost all patients develop recurring tumors, which have limited treatment choices and little hope of cure. The median survival is 15 months and tumor recurrence is inevitable. Only about 10% patient population survive longer than 3 years. Given the short life span of these patients, no effective treatment, there is a need to identify a priori the patient population with short survival, and identify the protein signatures that would guide to new drug targets. Thus, there is an ongoing need for biomarker identification to determine survival outcome, and develop new and efficient drugs to treat this lethal disease.

I have been studying GBM to identify protein signatures from tumor biopsies and plasma using MS-based proteomics approaches to i) identify GBM biomarkers ii) prognostic biomarkers for survival prediction (long term vs short term)  iii) predict response to bevacizumab in recurring tumors in GBM patients. Identifying specific inhibitors in these patients will open avenues to new treatment options in GBM. With this individualized approach, the physician can minimize the guesswork and time involved in finding a successful treatment. It is hoped this research will play a critical role in extending and improving the quality of life for these terminal cancer patients.

A rapid non-invasive urinary test for the detection of obstructive uropathy

Diseases of the urinary tract in newborns can lead to infant death, kidney failure, and in those who survive, to chronic kidney disease (CKD) and early heart disease in adulthood. Despite the availability of pre-natal maternal care and advanced imaging, obstructive diseases of the urinary tract account for the majority of cases of end stage kidney disease and consume 24% of health care expenditure in this segment of population. Most of these conditions do not cause symptoms and therefore are silent killers and go undetected. Current routine urinary testing is not sensitive enough to uncover these conditions. To address this issue and identify this severe condition in infants efficiently, we discovered urinary biomarkers of congenital obstructive uropathy focusing on human ureteropelvic junction obstruction (UPJO). Using a MS-based proteomics approach we have identified biomarkers of UPJO in infants. Furthermore, we are in the process of translating these biomarkers identified from sophisticated MS technology to an easy to use multiplexed protein array for a point-of-care application. The diagnosis of these serious conditions through the low cost, point of care screening diagnostic test followed by treatment, can lead to cure in a majority of newborns and infants with congenital diseases of the urinary tract.

Technology Development:

I have always been involved in technology development for the efficient analysis of various classes of compounds by mass spectrometry. One of the research interests in our laboratory is to develop novel MS-based technologies for the comprehensive characterization of various analytes ranging from small molecules like organic compounds and metabolites to large molecules like proteins, peptides and oligonucleotides. This is extended to my further interests in proteomics where I have developed several novel proteomics approaches for the better characterization of proteins including mapping posttranslational modifications, quantitative approaches, membrane protein identification and other MS-based proteomics approaches.

Selected Publications

Uyar, D S., Huang, Y W., Chesnik, M A., Doan, N B., and Shama, Mirza P.“Comprehensive serum proteomic analysis in early endometrial cancer” Journal of proteomics234. (2021): 104099.
Rajaratnam, V, Islam, M M., Yang, M, Slaby, R, Ramirez, H M., and Shama, Mirza P.“Glioblastoma: Pathogenesis and Current Status of Chemotherapy and Other Novel Treatments” Cancers12.4 (2020): 937.
Stetson, L C., Ostrom, Q T., Schlatzer, D, Liao, P, Devine, K, Waite, K, Couce, M E., Harris PLR, , Kerstetter-Fogle, A, Berens, M E., Sloan, A E., Islam, M M., Rajaratnam, V, Shama, Mirza P., Chance, M R., and Barnholtz-Sloan, J S.“Proteins inform survival-based differences in patients with glioblastoma” Neuro-oncology advances2.1 (2020): vdaa039.
Yacout, S M., McIlwain, K L., Shama, Mirza P., and Gaillard, E R.“Characterization of Retinal Pigment Epithelial Melanin and Degraded Synthetic Melanin Using Mass Spectrometry and In Vitro Biochemical Diagnostics” Photochemistry and photobiology95.1 (2019): 183-191.
Ciezki, K, Wesener, S, Jaber, D, Shama, Mirza P., and Forst, Steven A.“ngrA-dependent natural products are required for interspecies competition and virulence in the insect pathogenic bacterium Xenorhabdus szentirmaii” Microbiology (Reading, England)165.5 (2019): 538-553.
Labbe, B D., Hall, C L., Kellogg, S L., Chen, Y, Koehn, O, Pickrum, A M., Shama, Mirza P., and Kristich, C J.“Reciprocal Regulation of PASTA Kinase Signaling by Differential Modification” Journal of bacteriology201.10 (2019): e00016-19.
Zhang, Q, Lee, S B., Chen, X, Stevenson, M E., Pan, J, Xiong, D, Zhou, Y, Miller, M S., Lubet, R A., Wang, Y, Shama, Mirza P., and You, M. “Optimized Bexarotene Aerosol Formulation Inhibits Major Subtypes of Lung Cancer in Mice” Nano letters19.4 (2019): 2231-2242.
Alhajala, H S., Nguyen, H S., Shabani, S, Best, B, Kaushal, M, Al-Gizawiy, M M., Erin Ahn, E Y., Knipstein, J A., Shama, Mirza P., Schmainda, K M., Chitambar, C R., and Doan, N B.“Irradiation of pediatric glioblastoma cells promotes radioresistance and enhances glioma malignancy via genome-wide transcriptome changes” Oncotarget9.75 (2018): 34122-34131.
Doan, N B., Nguyen, H S., Alhajala, H S., Jaber, B, Al-Gizawiy, M M., Ahn, E E., Mueller, W M., Chitambar, C R., Shama, Mirza P., and Schmainda, K M.“Identification of radiation responsive genes and transcriptome profiling via complete RNA sequencing in a stable radioresistant U87 glioblastoma model” Oncotarget9.34 (2018): 23532-23542.
Doan, Ninh B., Nguyen, Ha S., Montoure, Andrew, Al-Gizawiy, Mona M., Mueller, Wade M., Kurpad, Shekar, Rand, Scott D., Connelly, Jennifer M., Chitambar, Christopher R., Schmainda, Kathleen M., Mirza, Shama P., and . “Acid ceramidase is a novel drug target for pediatric brain tumors” Oncotarget8.15 (2017): 24753-24761.
Raphael, Roseanne, Purushotham, Diana, Gastonguay, Courtney, Chesnik, Marla A., Kwok, Wai-Meng, Wu, Hsiang-En, Shah, Sanjiv J., Mirza, Shama P., and Strande, Jennifer L.“Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction” Journal of translational medicine14.1 (2016): 18.
Cossette, S M., Bhute, V J., Bao, X, Harmann, L M., Horswill, M A., Sinha, I, Gastonguay, A, Pooya, S, Bordas, M, Kumar, S N., Shama, Mirza P., Palecek, S P., Strande, J L., and Ramchandran, R. “Sucrose Nonfermenting-Related Kinase Enzyme-Mediated Rho-Associated Kinase Signaling is Responsible for Cardiac Function” Circulation. Cardiovascular genetics9.6 (2016): 474-486.
Heroux, Maxime S., Chesnik, Marla A., Halligan, Brian D., Al-Gizawiy, Mona, Connelly, Jennifer M., Mueller, Wade M., Rand, Scott D., Cochran, Elizabeth J., LaViolette, Peter S., Malkin, Mark G., Schmainda, Kathleen, and Shama, Mirza P.“Comprehensive characterization of glioblastoma tumor tissues for biomarker identification using mass spectrometry-based label-free quantitative proteomics” Physiological genomics46.13 (2014): 467–481.
Qi, Xiaomei, Xie, Congying, Hou, Songwang, Li, Gang, Yin, Ning, Dong, Lei, Lepp, Adrienne, Chesnik, Marla A., Mirza, Shama P., Szabo, Aniko, and others, . “Identification of a ternary protein-complex as a therapeutic target for K-Ras-dependent colon cancer” Oncotarget5.12 (2014): 4269-4282.
Alexanian, Anna, Miller, Bradley, Chesnik, Marla, Mirza, Shama, and Sorokin, Andrey. “Post-translational regulation of COX2 activity by FYN in prostate cancer cells” Oncotarget5.12 (2014): 4232–4243.
Bigley, Tarin M., Reitsma, Justin M., Mirza, Shama P., and Terhune, Scott S.“Human cytomegalovirus pUL97 regulates the viral major immediate early promoter by phosphorylation-mediated disruption of histone deacetylase 1 binding” Journal of virology87.13 (2013): 7393–7408.
Mesrobian, Hrair-George O., Kryger, John V., Groth, Travis W., Fiscus, Gabriel E., and Mirza, Shama P.“Urinary proteome analysis in patients with stable SFU grade 4 ureteropelvic junction obstruction differs from normal” Urology82.3 (2013): 745–e1.
Mesrobian, Hrair-George O., and Mirza, Shama P.“Hydronephrosis: a view from the inside” Pediatric clinics of North America59.4 (2012): 839–851.
Mirza, Shama P.“Quantitative mass spectrometry-based approaches in cardiovascular research” Circulation: Cardiovascular Genetics5.4 (2012): 477–483.
Narayan, Malathi, Mirza, Shama P., and Twining, Sally S.“Identification of phosphorylation sites on extracellular corneal epithelial cell maspin” Proteomics11.8 (2011): 1382–1390.