Marius Schmidt

 KEN 3087



Professor Schmidt is using physical methods to investigate biological molecules. He is concentrating mainly on static and time-resolved macromolecular crystallography to investigate the structure, dynamics and kinetics of proteins. Schmidt is also developing new, sophisticated software for the analysis of the time-resolved (4-dimensional) X-ray data. Results from crystallography are corroborated by (time or energy resolved) spectroscopic methods.

Biographical Sketch

Marius Schmidt obtained his Dipl. in Biology in 1990 at Johannes-Gutenberg-University, Mainz (Germany). He received his Dr. rer. Nat. in 1996 at Technische Universität München, Garching (Germany), where later he became habilitated in Experimental Physics in 2004. In 2008 he obtained an habilitation in the French system in Theoretical, physical and analytical Chemistry.

From 1996 to 1998 Schmidt worked as a scientist for the Physics Department at Technische Universität München (Germany) and from 1998 to 2001 as a research associate for the Department of Biochemistry and Molecular Biology at the University of Chicago. From 2001 to 2007 he worked as a scientist and Privatdozent for the Physics Department at Technische Universität München (TUM), (Germany).

Schmidt has been at the University of Wisconsin-Milwaukee since 2007, where he holds the rank of Professor.

Selected Publications

Schwander, Peter, and Schmidt, Marius. Mumbo Jumbo. 2025.
Poudyal, Ishwor, Schmidt, Marius, and Schwander, Peter. “Single-Particle Imaging by X-Ray Free-Electron Lasers – How Many Snapshots are Needed?.” Structural Dynamics 7.2 (2020): 12.
Gisriel, Chris, Coe, Jesse, Letrun, Romain, Yefanov, Oleksandr M., Luna-Chavez, Cesar, Stander, Natasha E., Lisova, Stella, Mariani, Valerio, Kuhn, Manuela, Aplin, Steve, Grant, Thomas D., Doerner, Katerina, Sato, Tokushi, Echelmeier, Austin, Villarreal, Jorvani C., Hunter, Mark S., Wiedorn, Max O., Knoska, Juraj, Mazalova, Victoria, Roy-Chowdhury, Shatabdi, Yang, Jay-How, Jones, Alex, Bean, Richard, Bielecki, Johan, Kim, Yoonhee, Mills, Grant, Weinhausen, Britta, Meza, Jose D., Al-Qudami, Nasser, Bajt, Sasa, Brehm, Gerrit, Botha, Sabine, Boukhelef, Djelloul, Brockhauser, Sandor, Bruce, Barry D., Coleman, Matthew A., Danilevski, Cyril, Discianno, Erin, Dobson, Zachary, Fangohr, Hans, Martin-Garcia, Jose M., Gevorkov, Yaroslav, Hauf, Steffen, Hosseinizadeh, Ahmad, Januschek, Friederike, Ketawala, Gihan K., Kupitz, Christopher, Maia, Luis, Manetti, Maurizio, Messerschmidt, Marc, Michelat, Thomas, Mondal, Jyotirmoy, Ourmazd, Abbas, Previtali, Gianpietro, Sarrou, Iosifina, Schoen, Silvan, Schwander, Peter, Shelby, Megan L., Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Szuba, Janusz, Turcato, Monica, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Abdellatif, Mohamed H., Zook, James D., Spence, John C. H., , Chapman, Henry N., Barty, Anton, Kirian, Richard A., Frank, Matthias, Ros, Alexandra, Schmidt, Marius, Fromme, Raimund, Mancuso, Adrian P., Fromme, Petra, and Zatsepin, Nadia A. “Membrane protein megahertz crystallography at the European XFEL.” NATURE COMMUNICATIONS 10. (2019).
Sanchez, J C., Carrillo, M, Pandey, S, Noda, M, Aldama, L, Feliz, D, Claesson, E, Wahlgren, W Y., Tracy, G, Duong, P, Nugent, A C., Field, A, Šrajer, V, Kupitz, C, Iwata, S, Nango, E, Tanaka, R, Tanaka, T, Fangjia, L, Tono, K, Owada, S, Westenhoff, S, Schmidt, Marius, and Stojković, E A. “High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures.” Structural dynamics (Melville, N.Y.) 6.5 (2019): 054701.
Niessen, Katherine A., Xu, Mengyang, George, Deepu K., Chen, Michael C., Ferre-D’Amare, Adrian R., Snell, Edward H., Cody, Vivian, Pace, James, Schmidt, Marius, and Markelz, Andrea G. “Protein and RNA dynamical fingerprinting.” NATURE COMMUNICATIONS 10. (2019).
Woitowich, N C., Halavaty, A S., Waltz, P, Kupitz, C, Valera, J, Tracy, G, Gallagher, K D., Claesson, E, Nakane, T, Pandey, S, Nelson, G, Tanaka, R, Nango, E, Mizohata, E, Owada, S, Tono, K, Joti, Y, Nugent, A C., Patel, H, Mapara, A, Hopkins, J, Duong, P, Bizhga, D, Kovaleva, S E., St Peter, R, Hernandez, C N., Ozarowski, W B., Roy-Chowdhuri, S, Edlund, P, Takala, H, Ihalainen, J, Brayshaw, J, Norwood, T, Poudyal, I, Fromme, P, Spence JCH, , Moffat, K, Westenhoff, S, Schmidt, Marius, and Stojković, E A. “Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome.” IUCrJ 5.Pt 5 (2018): 619-634.
Olmos, Jr, J L., Pandey, S, Martin-Garcia, J M., Calvey, G, Katz, A, Knoska, J, Kupitz, C, Hunter, M S., Liang, M, Oberthuer, D, Yefanov, O, Wiedorn, M, Heyman, M, Holl, M, Pande, K, Barty, A, Miller, M D., Stern, S, Roy-Chowdhury, S, Coe, J, Nagaratnam, N, Zook, J, Verburgt, J, Norwood, T, Poudyal, I, Xu, D, Koglin, J, Seaberg, M H., Zhao, Y, Bajt, S, Grant, T, Mariani, V, Nelson, G, Subramanian, G, Bae, E, Fromme, R, Fung, R, Schwander, Peter, Frank, M, White, T A., Weierstall, U, Zatsepin, N, Spence, J, Fromme, P, Chapman, H N., Pollack, L, Tremblay, L, Ourmazd, Abbas, Phillips, Jr, G N., and Schmidt, Marius. “Enzyme intermediates captured ‘on the fly’ by mix-and-inject serial crystallography.” BMC biology 16.1 (2018): 59.
Hosseinizadeh, Achmad, Mashayekhi, Goncheh, Copperman, Jeremy, Schwander, Peter, Dashti, Ali, Sepehr, Reyhaneh, Fung, Russel, Schmidt, Marius, Yoon, Chuck, Hogue, Brenda, Williams, Garth J., Aquilla, Andy, and Ourmazd, Abbas. “Conformational Landscape of a Virus by Single-particle X-ray Scattering.” Nature Methods online. (2017).
Schmidt, Marius. “Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources.” Methods in molecular biology (Clifton, N.J.) 1607. (2017): 273-294.
Kupitz, Christopher, Jose, Olmos, Holl, Mark, Tremblay, Lee, Pande, Kanupriya, Pandey, Suraj, , , , , , , , Schmidt, Marius, and . “Structural enzymology using X-ray free electron lasers.” Structural Dynamics. (2016).
Pande, Kanupriya, Hutchinson, Christopher D., Groenhof, Gerrit, Aquilla, Andy, Robinson, Joe S., Tenboer, Jason, Basu, Shibom, DePonte, Daniel D., Liang, Meng, White, Thomas, Zatsepin, Nadia A., Yefanov, Oleksandr, Morozov, Dimitry, Oberthuer, Dominik, Gati, Cornelius, Subramanian, Ganesh, James, Daniel, Zhao, Yun, Koralek, Jacob, Brayshaw, Jennifer, Kupitz, Christopher, Conrad, Chelsie, Roy-Showdhuri, Shatabdi, Coe, Jesse D., Metz, Markus, Xavier, Poulray L., Grant, Thomas E., Koglin, Jason E., Ketawala, Gihan, Fromme, Raimund, Srajer, Vukica, Henning, Robert, Spence, John C., Ourmazd, Abbas, Schwander, Peter, Weierstall, Uwe, Frank, Matthias, Fromme, Petra, Barty, Anton, Chapman, Henry N., Moffat, Keith, van Thor, Jasper J., and Schmidt, Marius. “Femtosecond Structural Dynamics Drives the Trans/Cis Isomerization in Photoactive Yellow Protein.” Science 352. (2016): 725-729.
Ayyer, K, Yefanov, O M., Oberthür, D, Roy-Chowdhury, S, Galli, L, Mariani, V, Basu, S, Coe, J, Conrad, C E., Fromme, R, Schaffer, A, Dörner, K, James, D, Kupitz, C, Metz, M, Nelson, G, Xavier, P L., Beyerlein, K R., Schmidt, Marius, Sarrou, I, Spence, J C., Weierstall, U, White, T A., Yang, J H., Zhao, Y, Liang, M, Aquila, A, Hunter, M S., Robinson, J S., Koglin, J E., Boutet, S, Fromme, P, Barty, A, and Chapman, H N. “Macromolecular diffractive imaging using imperfect crystals.” Nature 530.7589 (2016): 202-6.
Edlund, P, Takala, H, Claeson, E, Poudyal, I, Pandey, S, Kupitz, C, Stojkovic, E, Schmidt, Marius, and Westenhoff, S. “The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography.” Scientific Reports. (2016).
Schmidt, Marius, Pande, Kanupriya, Basu, Shibom, and Tenboer, Jason. “Room Temperature Structures beyond 1.5 Å by Serial Femtosecond Crystallography.” Structural Dynamics 2.041708 (2015): 1-9.
Pande, Kanupriya, Schmidt, Marius, Schwander, Peter, and Saldin, Dilano. “Simulations on time-resolved structure determination of uncrystallized biomolecules in the presence of shot noise.” STRUCTURAL DYNAMICS 2. (2015).
Tenboer, Jason, Basu, Shibom, Zatsepin, Nadia, Pande, Kanupriya, Milathianaki, Despina, Frank, Matthias, Hunter, Mark, Boutet, Sebastien, Williams, Garth, Koglin, Jason E., Oberthuer, Dominik, Heymann, Michael, Kupitz, Chris, Conrad, Chelsie, Coe, Jesse, Roy-Chowdhury, Shatabti, Weierstall, Uwe, James, Dan, Wang, Dingjie, Grant, Tom, Barty, Anton, Yefanov, Oleksandr, Scales, Jennifer, Gati, Cornelius, Seuring, Carolin, Srajer, Vukica, Henning, Robert, Schwander, Peter, Fromme, Raimund, Ourmazd, Abbas, Moffat, Keith, Van Thor, Jasper J., Spence, John C., Fromme, Petra, Chapman, Henry, and Schmidt, Marius. “Time Resolved Serial Crystallography Captures High Resolution Intermediates of Photoactive Yellow Protein.” Science 346.6214 (2014).
Schmidt, Marius, and Saldin, Dilano K. “Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer.” Structural Dynamics 1. (2014): 024701, 1-14.
Malik, Vikash, Sen, Somaditya, Gelting, David R., Gajdardziska-Josifovska, Marija, Schmidt, Marius, and Guptasarma, Prasenjit. “Field-enhanced magnetic moment in ellipsoidal nanohematite.” MATERIALS RESEARCH EXPRESS 1.2 (2014).
Schmidt, Marius, Srajer, Vukica, Ihee, Harry, Henning, Robert, Purwar, Namrta, Tenboer, Jason, and Tripathi, Shailesh. “Protein Energy Landscape Determined by Five-Dimensional Crystallography.” Acta Crystallographica D 69. (2013): 2534-2542.
Purwar, N, Tenboer, J, Tripathi, S, and Schmidt, Marius. “Spectroscopic studies of model photo-receptors: Validation of a nanosecond time-resolved micro-spectrophotometer design using photoactive yellow protein and α – phycoerythrocyanin.” International Journal of Molecular Sciences 14. (2013): 18881-18898.
Schmidt, Marius. “Mix and Inject: Reaction Initiation by Diffusion for Time-Resolved Macromolecular Crystallography.” Advances on Condensed Matter Physics 2013. Ed. Ormandy, Alex O. (2013): 1-10.
Jung, Y.O., Lee, H., Kim, J, Schmidt, Marius, Moffat, K., Srajer, V., and Ihee, H. “Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography.” Nature Chemistry 5.212-220 (2013).
Youngblut, Matt, Judd, Evan, Srajer, Vukica, Sayyed, Bilal, Goelzer, Tyler, Elliot, Sean, Schmidt, Marius, and Pacheco, Andy. “Laue Structure of Shewanella oneidensis ccNIR.” Journal of Biological Inorganic Chemistry 17. (2012): 647-672.
Schmidt, Marius, Srajer, Vukica, Purwar, Namrta, and Tripathi, Shailesh. “The Kinetic Dose Limit in Room-Temperature Time-Resolved Laue Crystallography.” J. Synchrotron Rad. 19.2 (2012): 264-273.
Tripathi, Shailesh, Srajer, Vukica, Purwar, Namrta, Henning, Robert, and Schmidt, Marius. “The pH dependence of the PYP photocycle – a time-resolved crystallographic investigation.” Biophysics Journal 102. (2012): 325-332.
Purwar, Namrta, McGarry, Jennifer, Kostera, Josh, Pacheco, Andy, and Schmidt, Marius. “Interaction of Nitric Oxide with Catalase: Structural, and Kinetic Analysis.” Biochemistry 50. (2011): 4491-4503.
Schmidt, Marius, Graber, Tim, Henning, Rob, and Srajer, Vukica. “Five-Dimensional Crystallography.” Acta Cryst A 66. (2010): 198-206.
Schmidt, Marius, Patel, A., Zhao, Y., and Reuter, W. “Structural basis for the photochemistry of alfa-phycoerythrocyanin.” Biochemistry 46. (2007): 416-423.