Marius Schmidt
- Professor, Physics
Research Interests
Professor Schmidt is using physical methods to investigate biological molecules. He is concentrating mainly on static and time-resolved macromolecular crystallography to investigate the structure, dynamics and kinetics of proteins. Schmidt is also developing new, sophisticated software for the analysis of the time-resolved (4-dimensional) X-ray data. Results from crystallography are corroborated by (time or energy resolved) spectroscopic methods.
Biographical Sketch
Marius Schmidt obtained his Dipl. in Biology in 1990 at Johannes-Gutenberg-University, Mainz (Germany). He received his Dr. rer. Nat. in 1996 at Technische Universität München, Garching (Germany), where later he became habilitated in Experimental Physics in 2004. In 2008 he obtained an habilitation in the French system in Theoretical, physical and analytical Chemistry.
From 1996 to 1998 Schmidt worked as a scientist for the Physics Department at Technische Universität München (Germany) and from 1998 to 2001 as a research associate for the Department of Biochemistry and Molecular Biology at the University of Chicago. From 2001 to 2007 he worked as a scientist and Privatdozent for the Physics Department at Technische Universität München (TUM), (Germany).
Schmidt has been at the University of Wisconsin-Milwaukee since 2007, where he holds the rank of Professor.
Selected Publications
Hosseinizadeh, achmad, Breckwold, N, Fung, Russell, Schmidt, Marius, Schwander, Peter, Santra, R., and Ourmazd, Abbas. “Single-femtosecond atomic-resolution observation of a protein traversing a conical intersection” Nature(2021).
Schmidt, Marius. “Macromolecular Movies, Storybooks Written by Nature” Biophysical Reviews(2021).
Carrillo, M, Pandey, S, Sanchez, J, Noda, M, Poudyal, I, Aldama, L, Malla, T N., Claesson, E, Wahlgren, W Y., Feliz, D, Šrajer, V, Maj, M, Castillon, L, Iwata, S, Nango, E, Tanaka, R, Tanaka, T, Fangjia, L, Tono, K, Owada, S, Westenhoff, S, Stojković, E A., and Schmidt, Marius. “High-resolution crystal structures of transient intermediates in the phytochrome photocycle” Structure (London, England : 1993)(2021).
Yun, J H., Li , X, Yue, J, Park, J H., Jin, Z, Li , C, Hu, H, Shi, Y, Pandey, S, Carbajo, S, Boutet, S, Hunter, M S., Liang, M, Sierra, R G., Lane, T J., Zhou, L, Weierstall, U, Zatsepin, N A., Ohki, M, Tame JRH, , Park, S Y., Spence JCH, , Zhang, W, Schmidt, Marius, Lee, W, and Liu, H. “Early-stage dynamics of chloride ion-pumping rhodopsin revealed by a femtosecond X-ray laser” Proceedings of the National Academy of Sciences of the United States of America118.13 (2021).
Claesson, E, Wahlgren, W Y., Takala, H, Pandey, S, Castillon, L, Kuznetsova, V, Henry, L, Panman, M, Carrillo, M, Kübel, J, Nanekar, R, Isaksson, L, Nimmrich, A, Cellini, A, Morozov, D, Maj, M, Kurttila, M, Bosman, R, Nango, E, Tanaka, R, Tanaka, T, Fangjia, L, Iwata, S, Owada, S, Moffat, K, Groenhof, G, Stojković, E A., Ihalainen, J A., Schmidt, Marius, and Westenhoff, S. “The primary structural photoresponse of phytochrome proteins captured by a femtosecond X-ray laser” eLife9. (2020).
Schmidt, Marius. “Reaction Initiation in Enzyme Crystals by Diffusion of Substrate” Crystals(2020).
Gisriel, Chris, Coe, Jesse, Letrun, Romain, Yefanov, Oleksandr M., Luna-Chavez, Cesar, Stander, Natasha E., Lisova, Stella, Mariani, Valerio, Kuhn, Manuela, Aplin, Steve, Grant, Thomas D., Doerner, Katerina, Sato, Tokushi, Echelmeier, Austin, Villarreal, Jorvani C., Hunter, Mark S., Wiedorn, Max O., Knoska, Juraj, Mazalova, Victoria, Roy-Chowdhury, Shatabdi, Yang, Jay-How, Jones, Alex, Bean, Richard, Bielecki, Johan, Kim, Yoonhee, Mills, Grant, Weinhausen, Britta, Meza, Jose D., Al-Qudami, Nasser, Bajt, Sasa, Brehm, Gerrit, Botha, Sabine, Boukhelef, Djelloul, Brockhauser, Sandor, Bruce, Barry D., Coleman, Matthew A., Danilevski, Cyril, Discianno, Erin, Dobson, Zachary, Fangohr, Hans, Martin-Garcia, Jose M., Gevorkov, Yaroslav, Hauf, Steffen, Hosseinizadeh, Ahmad, Januschek, Friederike, Ketawala, Gihan K., Kupitz, Christopher, Maia, Luis, Manetti, Maurizio, Messerschmidt, Marc, Michelat, Thomas, Mondal, Jyotirmoy, Ourmazd, Abbas, Previtali, Gianpietro, Sarrou, Iosifina, Schoen, Silvan, Schwander, Peter, Shelby, Megan L., Silenzi, Alessandro, Sztuk-Dambietz, Jolanta, Szuba, Janusz, Turcato, Monica, White, Thomas A., Wrona, Krzysztof, Xu, Chen, Abdellatif, Mohamed H., Zook, James D., Spence, John C. H., , Chapman, Henry N., Barty, Anton, Kirian, Richard A., Frank, Matthias, Ros, Alexandra, Schmidt, Marius, Fromme, Raimund, Mancuso, Adrian P., Fromme, Petra, and Zatsepin, Nadia A.“Membrane protein megahertz crystallography at the European XFEL” NATURE COMMUNICATIONS10. (2019).
Sanchez, J C., Carrillo, M, Pandey, S, Noda, M, Aldama, L, Feliz, D, Claesson, E, Wahlgren, W Y., Tracy, G, Duong, P, Nugent, A C., Field, A, Šrajer, V, Kupitz, C, Iwata, S, Nango, E, Tanaka, R, Tanaka, T, Fangjia, L, Tono, K, Owada, S, Westenhoff, S, Schmidt, Marius, and Stojković, E A.“High-resolution crystal structures of a myxobacterial phytochrome at cryo and room temperatures” Structural dynamics (Melville, N.Y.)6.5 (2019): 054701.
Schmidt, Marius. “Time-resolved macromolecular crystallography at pulsed X-ray sources” IJMS(2019).
Niessen, Katherine A., Xu, Mengyang, George, Deepu K., Chen, Michael C., Ferre-D'Amare, Adrian R., Snell, Edward H., Cody, Vivian, Pace, James, Schmidt, Marius, and Markelz, Andrea G.“Protein and RNA dynamical fingerprinting” NATURE COMMUNICATIONS10. (2019).
Woitowich, N C., Halavaty, A S., Waltz, P, Kupitz, C, Valera, J, Tracy, G, Gallagher, K D., Claesson, E, Nakane, T, Pandey, S, Nelson, G, Tanaka, R, Nango, E, Mizohata, E, Owada, S, Tono, K, Joti, Y, Nugent, A C., Patel, H, Mapara, A, Hopkins, J, Duong, P, Bizhga, D, Kovaleva, S E., St Peter, R, Hernandez, C N., Ozarowski, W B., Roy-Chowdhuri, S, Edlund, P, Takala, H, Ihalainen, J, Brayshaw, J, Norwood, T, Poudyal, I, Fromme, P, Spence JCH, , Moffat, K, Westenhoff, S, Schmidt, Marius, and Stojković, E A.“Structural basis for light control of cell development revealed by crystal structures of a myxobacterial phytochrome” IUCrJ5.Pt 5 (2018): 619-634.
Olmos, Jr, J L., Pandey, S, Martin-Garcia, J M., Calvey, G, Katz, A, Knoska, J, Kupitz, C, Hunter, M S., Liang, M, Oberthuer, D, Yefanov, O, Wiedorn, M, Heyman, M, Holl, M, Pande, K, Barty, A, Miller, M D., Stern, S, Roy-Chowdhury, S, Coe, J, Nagaratnam, N, Zook, J, Verburgt, J, Norwood, T, Poudyal, I, Xu, D, Koglin, J, Seaberg, M H., Zhao, Y, Bajt, S, Grant, T, Mariani, V, Nelson, G, Subramanian, G, Bae, E, Fromme, R, Fung, R, Schwander, Peter, Frank, M, White, T A., Weierstall, U, Zatsepin, N, Spence, J, Fromme, P, Chapman, H N., Pollack, L, Tremblay, L, Ourmazd, Abbas, Phillips, Jr, G N., and Schmidt, Marius. “Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography” BMC biology16.1 (2018): 59.
Schmidt, Marius. “Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources” Methods in molecular biology (Clifton, N.J.)1607. (2017): 273-294.
Schmidt, Marius. “A short history of structure based research on the photocycle of photoactive yellow protein” Structural Dynamics(2017).
Hosseinizadeh, Achmad, Mashayekhi, Goncheh, Copperman, Jeremy, Schwander, Peter, Dashti, Ali, Sepehr, Reyhaneh, Fung, Russel, Schmidt, Marius, Yoon, Chuck, Hogue, Brenda, Williams, Garth J., Aquilla, Andy, and Ourmazd, Abbas. “Conformational Landscape of a Virus by Single-particle X-ray Scattering” Nature Methodsonline. (2017).
Kupitz, Christopher, Jose, Olmos, Holl, Mark, Tremblay, Lee, Pande, Kanupriya, Pandey, Suraj, , , , , , , , Schmidt, Marius, and . “Structural enzymology using X-ray free electron lasers” Structural Dynamics(2016).
Edlund, P, Takala, H, Claeson, E, Poudyal, I, Pandey, S, Kupitz, C, Stojkovic, E, Schmidt, Marius, and Westenhoff, S. “The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography” Scientific Reports(2016).
Pande, Kanupriya, Hutchinson, Christopher D., Groenhof, Gerrit, Aquilla, Andy, Robinson, Joe S., Tenboer, Jason, Basu, Shibom, DePonte, Daniel D., Liang, Meng, White, Thomas, Zatsepin, Nadia A., Yefanov, Oleksandr, Morozov, Dimitry, Oberthuer, Dominik, Gati, Cornelius, Subramanian, Ganesh, James, Daniel, Zhao, Yun, Koralek, Jacob, Brayshaw, Jennifer, Kupitz, Christopher, Conrad, Chelsie, Roy-Showdhuri, Shatabdi, Coe, Jesse D., Metz, Markus, Xavier, Poulray L., Grant, Thomas E., Koglin, Jason E., Ketawala, Gihan, Fromme, Raimund, Srajer, Vukica, Henning, Robert, Spence, John C., Ourmazd, Abbas, Schwander, Peter, Weierstall, Uwe, Frank, Matthias, Fromme, Petra, Barty, Anton, Chapman, Henry N., Moffat, Keith , van Thor, Jasper J., and Schmidt, Marius. “Femtosecond Structural Dynamics Drives the Trans/Cis Isomerization in Photoactive Yellow Protein” Science352. (2016): 725-729.
Ayyer, K, Yefanov, O M., Oberthür, D, Roy-Chowdhury, S, Galli, L, Mariani, V, Basu, S, Coe, J, Conrad, C E., Fromme, R, Schaffer, A, Dörner, K, James, D, Kupitz, C, Metz, M, Nelson, G, Xavier, P L., Beyerlein, K R., Schmidt, Marius, Sarrou, I, Spence, J C., Weierstall, U, White, T A., Yang, J H., Zhao, Y, Liang, M, Aquila, A, Hunter, M S., Robinson, J S., Koglin, J E., Boutet, S, Fromme, P, Barty, A, and Chapman, H N.“Macromolecular diffractive imaging using imperfect crystals” Nature530.7589 (2016): 202-6.
Schmidt, Marius, Pande, Kanupriya, Basu, Shibom, and Tenboer, Jason. “Room Temperature Structures beyond 1.5 Å by Serial Femtosecond Crystallography” Structural Dynamics2.041708 (2015): 1-9.