Professor Joachim Frank, Columbia University

New Opportunities in Single-particle Cryo-EM: Mapping States in an Ensemble, Trapping Short-lived States

Single-particle cryo-EM provides experimental access to large ensembles of biological molecules, but current methods of analysis fall short of mining the rich information buried in the data sets. In addition, the length of time of sample preparation in the normal experimental setup precludes the investigation of short-lived states. New methods of data analysis pioneered in a collaboration with Abbas Ourmazd’s group, and new experimental methods for trapping of states with a life time of 10-1000 ms both offer opportunities for research that is more function-oriented than current methodology.

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Additional seating avaialble in KIRC 1150