A. Milwaukee Symposium
Day 1: Monday, August 25, 2022
UW-Milwaukee, Kenwood IRC (KIRC), 3135 North Maryland Ave., Milwaukee,WI
| 07:45 – 08:45 | REGISTRATION (KIRC Atrium)
and BREAKFAST Food served in KIRC Atrium 1140 Seating in KIRC 1130 & 1150 |
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| 08:45 – 09:00 | OPENING & Welcome (KIRC 1150) | |
| SESSION 1 (KIRC Room 1150)
Chair: Graeme Milligan (University of Glasgow) |
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| 09:00 – 09:30 | Keynote 1
Catherine Royer (Rensselaer Polytechnic Institute) |
Mechanisms controlling Start in budding yeast revealed by fluctuation microscopy |
| 09:30 – 09:40 | Keynote 1 Q & A | |
| 09:40 – 10:10 | Keynote 2
Kai Zhang (University of Illinois at Urbana-Champaign) |
Finetune receptor tyrosine kinase activity with light |
| 10:10 – 10:20 | Keynote 2 Q & A | |
| 10:20 – 11:20 | Coffee Break and First Poster Viewing Session | |
| SESSION 2 (KIRC Room 1150)
Chair: Ionel Popa (University of Wisconsin-Milwaukee) |
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| 11:20 – 11:50 | Keynote 3
Suzanne Scarlata (Worcester Polytechnic Institute) |
Using fluorescence imaging to uncover novel G protein / phospholiphase C6 signaling pathways |
| 11:50 – 12:00 | Keynote 3 Q & A | |
| 12:00 – 12:30 | Selected Poster Talks (SPTs)
(5-minute talks & questions) David Frick (UW-Milwaukee) Tyler Camp (UI @ Urbana-Champaign) Monica Gonzalez-Magaldi (UT @ Austin) Madison Rennie (Worcester) Ece Ozdemir (Johns Hopkins) |
The SARS-CoV-2 Nsp3 Mac1 domain . . . Single-molecule orientation . . . Receptors for Insulin . . . Changes in stress granule formation . . . A quantitative microscopy method . . .
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12:30 – 13:30 |
LUNCH (Served in KIRC 1140)
Seating in KIRC 1130 and 1160 |
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Symposium, Day 1: Thursday, 25 August 2022 (continued)
UW-Milwaukee, Kenwood IRC (KIRC), 3135 North Maryland Ave., Milwaukee, WI
| SESSION 3 (KIRC 1150)
Chair: Claudiu Gradinaru (University of Toronto at Mississauga) |
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| 13:30 – 14:00 | Keynote 4
Kalina Hristova (Johns Hopkins University) |
Quantification of ligand-induced lipid and protein microdomains with distinct signaling properties |
| 14:00 – 14:10 | Keynote 4 Q & A | |
| 14:10 – 14:40 | Keynote 5
Ionel Popa (University of Wisconsin-Milwaukee) |
A live image processing algorithm to measure fluctuations of single molecules and maintain sample focus with nanometer resolution over hour-long recordings |
| 14:40 – 14:50 | Keynote 5 Q & A | |
| 14:50 – 15:40 | General Discussion
Instigated by: Kalina Hristova (Johns Hopkins) Graeme Milligan (Glasgow) |
What kind of microscopies do we have that people are not aware of? What kind of microscopy are we missing and what (realistic) features should it present? |
| 15:40 – 16:40 | Coffee Break and Second Poster Viewing Session | |
| SESSION 4 (KIRC 1150)
Chair: Vali Raicu (University of Wisconsin-Milwaukee) |
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| 16:40 – 17:10 | Keynote 6
Anne Kenworthy (University of Virginia School of Medicine) |
High-content imaging platform to discover chemical modulators of plasma membrane rafts |
| 17:10 – 17:20 | Keynote 6 Q & A | |
| 17:20 – 17:30 | Selected Poster Talks (SPTs)
(5-minute talks & questions) Sabita Sarma (UW-Milwaukee) Dammar Badu (UW-Milwaukee |
Talin R8 domain … response to force … Refinement of FRET spectrometry |
| 17:30 | Recombobulating and walking to Reception | |
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18:00 – 21:15 |
SYMPOSIUM RECEPTION and DINNER Location: The Zelazo Center for the Performing Arts
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21:30 |
Limousine shuttle departs for Hyatt Place |
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Symposium, Day 2: Friday, August 26, 2022
UW-Milwaukee, Kenwood IRC (KIRC), 3135 North Maryland Ave., Milwaukee, WI
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07:45 – 08:30 |
BREAKFAST |
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| SESSION 5 (KIRC Room 1150)
Chair: Anne Kenworthy (University of Virginia School of Medicine) |
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| 08:30 – 09:00 | Keynote 7
Graeme Milligan (University of Glasgow) |
The in-situ quaternary organisation of M1 muscarinic receptors |
| 09:00-09:10 | Keynote 7 Q & A | |
| 09:10-09:40 | Keynote 8
Michael Stoneman (University of Wisconsin-Milwaukee) |
Combination of FRET spectrometry and molecular dynamics simulations to probe receptor quaternary structure |
| 09:40 – 09:50 | Keynote 8 Q & A | |
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09:50 – 10:30 |
Coffee Break and Third Poster Viewing Session |
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| 10:30 – 11:10 | General Discussion
Instigated by: Catherine Royer (Rensselaer) Vali Raicu (UW-Milwaukee) |
A. What kind of errors do we encounter in fluorescence fluctuation spectroscopies?
B. What are some less-known errors in FRET? |
| SESSION 6 (KIRC Room 1150)
Chair: Kalina Hristova (Johns Hopkins University) |
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| 11:10 – 11:40 | Keynote 9
Claudiu Gradinaru (University of Toronto at Mississauga) |
Ligand-Base Conformations of the A2A Adenosine Receptor Revealed by Single-Molecule Fluorescence |
| 11:40 – 11:50 | Keynote 9 Q & A | |
| 11:50 – 12:20 | Keynote 10
Taras Pogorelov (University of Illinois at Urbana-Champaign)) |
Signaling through cellular membrane: capturing dynamic interactions of RTKs |
| 12:20 – 12:30 | Keynote 10 Q & A | |
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12:30 – 13:30 |
LUNCH
(Food served in KIRC 1140) Seating in KIRC 1130 and 1160
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B. RIIO Alliance 3rd General Assembly Meeting (Guests are welcome!)
Friday, August 26, 2022
UW-Milwaukee, Kenwood IRC (KIRC), 3135 North Maryland Ave., Milwaukee, WI
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RIIO ASSEMBLY Meeting of Members and Guests KIRC Room 1150
Chair: Graeme Milligan (University of Glasgow) |
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13:30 – 14:00 |
RIIOA Director Report Vali Raicu (University of Wisconsin-Milwaukee) |
Scientific and technology report |
| 14:00 – 14:10 | Q & A/Discussion | |
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14:10 – 14:40 |
Updates by Alliance Members Everyone is invited to bring a couple of slides with news to share |
Successful grant applications, high impact papers, new instruments planned, new methods, etc. |
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14:40 – 15:00 |
Oral Presentation Alexander Arnold (UW-Milwaukee) |
Drug Discovery in Milwaukee (MIDD) |
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15:00 – 15:30 |
Tour of the UWM Biophysical Microspectroscopy Facility (KIRC 2060) | |
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15:30 – 16:20 |
COFFEE and Planning/Brainstorming Session |
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| 16:20 –16:35 |
Closing Remarks |
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For the previous program information, please see the 2019 website