Wilcoxen, Jarett

Assistant Professor
Chemistry - Bio-Inorganic

B.S. in Biochemistry, University of California, Santa Barbara

Ph.D. in Biochemistry and Molecular Biology, University of California, Riverside (Russ Hille)

Post-Doctoral research associate, University of California-Davis (R. David Britt)



Research in the Wilcoxen lab will examine the structure-function relationship of metalloenzymes using biochemical and biophysical tools. We will explore, on a molecular level, how metalloenzymes generate highly reactive species, direct the reaction coordinate, and prevent unwanted side reactions. Researchers will develop skills in protein expression and purification, air free techniques with biological samples, spectroscopic techniques such as UV-vis and electron paramagnetic resonance spectroscopy.

Recent Publications:
16. Hu, S., Offenbacher, A., Thompson, E., Gee, C., Wilcoxen, J., Carr, C., Prigozhin, D., Yang, V., Alber, T., Britt, R.D., Fraser, J., Klinman, J.
Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The “Undoing” of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network
J. Am. Chem. Soc. 2019, 141, 1555-1567

15. Rettberg, L.A.*, Wilcoxen, J.*, Lee, C.C., Stiebritz, M.T., Tanifuji, K., Britt, R.D., and Hu, Y.
Probing the Coordination and Function of Fe4S4 Modules in Nitrogenase Assembly Protein NifB
Nature Communications 2018 9, 2824

14. Wilcoxen, J., Bruender, N.A., Bandarian, V., Britt, R.D.
A Radical Intermediate is Identified in QueE from Bacillus subtilis using 6-Carboxypterin as an Alternative Substrate.
J. Am. Chem. Soc. 2018, 140, 1753–1759

13. Lee, C.C., Wilcoxen, J., Hiller, C.J., Britt, R.D. and Hu, Y.
Evaluation of the Catalytic Relevance of the CO-bound States of V-Nitrogenase
Angew. Chem. Int. Ed. 2018, 57, 3411–3414

11. Dingwall, S., Wilcoxen, J., Niks, D., Hille, R.
Studies of Carbon Monoxide Dehydrogenase from Oligotropha carboxidovorans.
Journal of Molecular Catalysis B: Enzymatic. 2016, 134, 317-322

10. Wilcoxen, J.*, Arragain, S.*, Scandurra, A.A., Jimenez-Vicente, E., Echavarri-Erasun, C., Pollmann, S., Britt, R.D., and Rubio, L.M.
Electron Paramagnetic Resonance Characterization of Three Iron–Sulfur Clusters Present in the Nitrogenase Cofactor Maturase NifB from Methanocaldococcus infernus.
J. Am. Chem. Soc. 2016, 138, 7468-7471

9. Bruender, N.A., Wilcoxen, J., Britt, R.D., and Bandarian, V.
Biochemical and Spectroscopic Characterization of a Radical SAM Enzyme Involved in the Formation of a Peptide Thioether Crosslink.
Biochemistry. 2016, 55, 2122-2134

8. Zhu, W.*, Wilcoxen, J.*, Britt, R.D., and Richards, N.G.J.
Formation of Hexacoordinate Mn(III) in Bacillus subtilis Oxalate Decarboxylase Requires Catalytic Turnover.
Biochemistry. 2016, 55, 429-434

7. Hille, R., Dingwall, S., Wilcoxen, J.
The Aerobic CO Dehydrogenase from Oligotropha carboxidovorans.
J. Bio. Inorg. Chem. 2015 20, 243-251

6. Shanmugam, M., Wilcoxen, J., Habel-Rodriguez, D., Cutsail III, G. E., Kirk, M., Hoffman, B., and Hille, R.
13C and 63,65Cu ENDOR studies of CO dehydrogenase from Oligotropha carboxidovorans. Experimental Evidence in Support of a Copper-Carbonyl Intermediate.
J. Am. Chem. Soc. 2013, 135, 17775

5. Wilcoxen, J. and Hille, R.
Kinetic and Spectroscopic Studies of H2 Oxidation by the Mo/Cu containing Carbon Monoxide Dehydrogenase of Oligotropha carboxidovorans.
J. Biol. Chem. 2013, 288, 36052-36060

2. Wilcoxen, J., Snider, S., and Hille, R.
Substitution of Silver for Copper in the Binuclear Mo/Cu Center of Carbon Monoxide Dehydrogenase from Oligotropha carboxidovorans.
J. Am. Chem. Soc. 2011 133, 12934-12936