Project Description
Objectives: Synthesize novel ROS-activated prodrugs designed to release active drug species in response to reactive oxygen species (ROS). Characterize the chemical structure, purity, and stability of the prodrugs. Investigate the mechanism of prodrug activation and downstream biological effects in vitro.
Methodology: Synthesis: The student will assist in multi-step organic synthesis of ROS-responsive prodrugs using standard laboratory techniques under supervision. Characterization: Products will be characterized using NMR, LC-MS, HPLC, and UV-Vis spectroscopy to confirm structure, purity, and stability. Mechanistic Studies: In vitro assays will be used to evaluate ROS-dependent activation of the prodrugs, including monitoring release of active drug species under varying ROS conditions. Data Analysis: Reaction yields, spectroscopic data, and activation kinetics will be analyzed to understand structure–activity relationships.
Tasks and Responsibilites
Perform basic and advanced laboratory techniques such as weighing reagents, setting up reactions, and purifying products. Operate and interpret results from analytical instruments (NMR, LC-MS, HPLC). Maintain accurate lab notebooks and record all experimental observations. Assist in planning experiments and troubleshooting protocols under mentor guidance. Participate in group meetings and present findings in written and oral formats.
Desired Qualifications
None Listed.