Project Description
A deep understanding of image formation in optical microscopes is crucial for analyzing images and developing new imaging approaches. Research in this area focuses on the point spread function (PSF), which depicts the three-dimensional diffraction pattern of a point-like light source. The main objectives of our research are to develop a robust theoretical framework that accurately models the PSF, and to validate the theory experimentally. The SURF project aims to validate the simulations obtained from theory. Controlled experiments will be carried out using a two-pronged approach. First, a low-cost CCD camera mounted on a motorized xyz translation stage will be used to capture illumination PSF slices at various z-positions near the focus of our optical microscopy setup. These intensity images will reveal the PSF’s medium- and high-intensity features. Furthermore, we will use fluorescently labeled nano-beads as highly sensitive probes of lower-intensity features by scanning them across the focused illumination beam using the xyz translation stage. An ultrasensitive CCD camera will record the nanobead fluorescence, and the integrated intensity will be correlated with the bead position to reconstruct the 3D PSF.
Tasks and Responsibilites
Students will work together with my current graduate students to set up the lasers, align optics, set up the microscope, test the image acquisition software, and carry out the experiments. Students will receive safety training concerning all aspects of the experimental work, including the use of chemicals, electronic equipment, and lasers in the lab.