Project Description
Our objective is to develop clinically applicable strategies that integrate reactive oxygen species (ROS)-responsive prodrugs with selected pro-oxidants to enhance in vivo efficacy and tumor selectivity. Specifically, we will utilize arylboronate analogs of nitrogen mustard to evaluate the feasibility of this novel combination therapy. A series of pro-oxidants will be screened to identify candidates that most effectively synergize with ROS-activated prodrugs. We will assess the in vitro and in vivo antitumor efficacy and selectivity of the most promising combinations. In parallel, we will define the correlation between therapeutic efficacy and ROS levels in tumor tissues and evaluate the toxicological profiles of these treatments to determine their safety and translational potential.
Tasks and Responsibilites
The student will focus on evaluating the biological activity of ROS-responsive prodrugs in combination with pro-oxidants, with an emphasis on cytotoxicity studies and mechanistic investigation. Under the guidance of a graduate mentor, the student will culture cancer and normal epithelial cell lines, prepare treatment conditions, and perform cell viability assays (e.g., MTT or CellTiter-Glo) to assess anticancer efficacy and selectivity. In addition, the student will participate in fluorescence-based assays to monitor intracellular ROS levels and visualize drug activation in living cells. Mechanistic studies may also include apoptosis or DNA damage assays, depending on project progress. The student will maintain accurate experimental records, assist in data analysis, and present findings in lab meetings and internal symposia. Through this experience, the student will gain valuable hands-on training in cell biology, cancer pharmacology, and drug mechanism-of-action studies—building a strong foundation for future research or graduate study in biomedical sciences.
Desired Qualifications
None Listed.