Project Description
The objective of this project is to investigate how alternating pressure conditions influence mitochondria behavior and functional responses in cultured cells. The study will focus on observing changes in mitochondria activity and distribution while cells experience periodic pressure variations that mimic physiological or pathological mechanical environments. By examining how these fluctuating pressures alter cellular adhesion, gap formation, and overall mitochondrial health, the project aims to clarify the mechanisms by which mechanical stress regulates cellular function. The insights gained will contribute to a deeper understanding of pressure driven cellular responses and support the development of improved strategies for studying and managing conditions related to mechanical stress.
Tasks and Responsibilites
The student will design and fabricate microfluidic devices to culture endothelial cells or fibroblasts and establish stable cell monolayers suitable for controlled pressure studies. The student will prepare PDMS devices, apply appropriate surface treatments such as APTES, and perform perfusion culture to maintain healthy and confluent cells inside microchannels. Once cultures are ready, the student will operate the pressure pump system to expose cells to alternating pressure conditions that represent normal and hypertensive environments. The student will perform fluorescent staining to evaluate mitochondrial activity and adhesion proteins. The student will acquire fluorescence and confocal images, quantify cellular and mitochondrial responses, and compare results across different pressure patterns. Throughout the project, the student will maintain accurate experimental records, manage data analysis, and meet regularly with the mentor for progress updates. He will prepare a final report and presentation that summarize research methods, key findings, and conclusions.
Desired Qualifications
None Listed.