{"id":3443,"date":"2016-11-30T10:01:48","date_gmt":"2016-11-30T16:01:48","guid":{"rendered":"https:\/\/uwm.edu\/physics\/?post_type=tribe_events&p=3443"},"modified":"2016-11-30T10:07:42","modified_gmt":"2016-11-30T16:07:42","slug":"colloquium-ashish-mishra","status":"publish","type":"tribe_events","link":"https:\/\/uwm.edu\/physics\/event\/colloquium-ashish-mishra\/","title":{"rendered":"Colloquium: Ashish Mishra"},"content":{"rendered":"

This special Physics department colloquium is scheduled for Wednesday 11\/30\/16 at 2:00 PM in the Kenwood Interdisciplinary Research Complex (KIRC) Room KEN 2035. Anyone is welcome.<\/p>\n

Investigation of Membrane Receptors’ Oligomers Using Fluorescence Resonance Energy Transfer and Multiphoton Microscopy in Living Cells<\/strong>
\nAshish Mishra<\/strong>, PhD Candidate\/UWM Dept. of Physics<\/p>\n

Investigating quaternary structure (oligomerization) of macromolecules (such as proteins and nucleic acids) in living systems (in vivo) has been a great challenge in biophysics, due to molecular diffusion, fluctuations in several biochemical parameters such as pH, quenching of fluorescence by oxygen (when fluorescence methods are used), etc.<\/p>\n

We studied oligomerization of membrane receptors in living cells by means of Fluorescence (F\u00f6rster) Resonance Energy Transfer (FRET) using fluorescent markers and two photon excitation fluorescence micro-spectroscopy. Using suitable FRET models, we are able to determine the stoichiometry and quaternary structure of various macromolecular complexes. In this talk, I will present results of the above-mentioned study for two membrane receptors: (1) sigma-1 receptor and (2) rhodopsin, described below:<\/p>\n

1)\t Sigma-1 receptors are molecular chaperone proteins, which also regulate ion channels. S1R seems to be involved in substance abuse, as well as several diseases such as Alzheimer\u2019s.<\/p>\n

2)\t Rhodopsin is a prototypical G protein coupled receptor (GPCR) and is directly involved in vision. GPCRs form a large family of receptors that participate in cell signaling by responding to external stimuli such as drugs, thus being a major drug target (more than 40% drugs target GPCRs).<\/p>\n","protected":false},"excerpt":{"rendered":"

This special Physics department colloquium is scheduled for Wednesday 11\/30\/16 at 2:00 PM in the Kenwood Interdisciplinary Research Complex (KIRC) Room KEN 2035. Anyone is welcome. Investigation of Membrane Receptors’ Oligomers Using Fluorescence Resonance Energy Transfer and Multiphoton Microscopy in …<\/p>\n","protected":false},"author":63,"featured_media":0,"template":"","meta":{"_acf_changed":false,"_tribe_events_status":"","_tribe_events_status_reason":"","_tribe_events_is_hybrid":"","_tribe_events_is_virtual":"","_tribe_events_virtual_video_source":"","_tribe_events_virtual_embed_video":"","_tribe_events_virtual_linked_button_text":"","_tribe_events_virtual_linked_button":"","_tribe_events_virtual_show_embed_at":"","_tribe_events_virtual_show_embed_to":[],"_tribe_events_virtual_show_on_event":"","_tribe_events_virtual_show_on_views":"","_tribe_events_virtual_url":"","footnotes":"","uwm_wg_additional_authors":[]},"tags":[],"tribe_events_cat":[58],"class_list":["post-3443","tribe_events","type-tribe_events","status-publish","hentry","tribe_events_cat-physics-colloquia","cat_physics-colloquia"],"yoast_head":"\nPhysics & Astronomy<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/uwm.edu\/physics\/event\/colloquium-ashish-mishra\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Colloquium: Ashish Mishra\" \/>\n<meta property=\"og:description\" content=\"This special Physics department colloquium is scheduled for Wednesday 11\/30\/16 at 2:00 PM in the Kenwood Interdisciplinary Research Complex (KIRC) Room KEN 2035. 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