1.0Physics & Astronomyhttps://uwm.edu/physicsElle Cochranehttps://uwm.edu/physics/author/cochratcuwm-edu/rich600338<blockquote class="wp-embedded-content" data-secret="6j4tzrosoh"><a href="https://uwm.edu/physics/event/pqmarszalek041919/">Physics Colloquium – Piotr E. Marszalek</a></blockquote><iframe sandbox="allow-scripts" security="restricted" src="https://uwm.edu/physics/event/pqmarszalek041919/embed/#?secret=6j4tzrosoh" width="600" height="338" title="“Physics Colloquium – Piotr E. Marszalek” — Physics & Astronomy" data-secret="6j4tzrosoh" frameborder="0" marginwidth="0" marginheight="0" scrolling="no" class="wp-embedded-content"></iframe><script type="text/javascript"> /* <![CDATA[ */ /*! This file is auto-generated */ !function(d,l){"use strict";l.querySelector&&d.addEventListener&&"undefined"!=typeof URL&&(d.wp=d.wp||{},d.wp.receiveEmbedMessage||(d.wp.receiveEmbedMessage=function(e){var t=e.data;if((t||t.secret||t.message||t.value)&&!/[^a-zA-Z0-9]/.test(t.secret)){for(var s,r,n,a=l.querySelectorAll('iframe[data-secret="'+t.secret+'"]'),o=l.querySelectorAll('blockquote[data-secret="'+t.secret+'"]'),c=new RegExp("^https?:$","i"),i=0;i<o.length;i++)o[i].style.display="none";for(i=0;i<a.length;i++)s=a[i],e.source===s.contentWindow&&(s.removeAttribute("style"),"height"===t.message?(1e3<(r=parseInt(t.value,10))?r=1e3:~~r<200&&(r=200),s.height=r):"link"===t.message&&(r=new URL(s.getAttribute("src")),n=new URL(t.value),c.test(n.protocol))&&n.host===r.host&&l.activeElement===s&&(d.top.location.href=t.value))}},d.addEventListener("message",d.wp.receiveEmbedMessage,!1),l.addEventListener("DOMContentLoaded",function(){for(var e,t,s=l.querySelectorAll("iframe.wp-embedded-content"),r=0;r<s.length;r++)(t=(e=s[r]).getAttribute("data-secret"))||(t=Math.random().toString(36).substring(2,12),e.src+="#?secret="+t,e.setAttribute("data-secret",t)),e.contentWindow.postMessage({message:"ready",secret:t},"*")},!1)))}(window,document); //# sourceURL=https://uwm.edu/physics/wp-includes/js/wp-embed.min.js /* ]]> */ </script> Piotr Marszalek, Duke University Nanomechanics of Biopolymers Beyond Their Entropic Elasticity Regime Compared to other single-molecule techniques, AFM-based force spectroscopy uses stiff force transducers and it may apply large stretching forces to molecules, enabling their structural modifications and capturing high energy conformations that cannot be examined by (for example) X-ray crystallography or NMR. In my talk, I will present our AFM stretching and relaxation studies, supported by computer simulations of individual molecules of DNA and proteins.