Predictive Human Induced Pluripotent Stem Cell Derived Cardiomyocyte Model of Drug-Induced Cardiotoxicity

Rosy Joshi-Mukherjee
Aurora Research Institute / University of Wisconsin-Milwaukee
Research Scientist of Induced Pluripotent Stem Cells & Cardiomyocyte Biology

“Progress in cancer therapeutics has improved survival rates for most malignancies. However, chemotherapeutic agents can cause acute QT prolongation and cardiotoxicity. One of the major limitations in the field is to track the longitudinal functional drug response in the same cardiomyocyte network over time. Here, we report a novel platform for high-resolution field potential and action potential (AP) recordings from multiple human pluripotent stem cell derived cardiomyocyte constructs to screen drug associated cardiotoxicity. Micro-gold multielectrode arrays were used to electroporate multiple constructs simultaneously to obtain high throughput AP measurements. Our model has the capability to distinguish subtle differences in AP morphology to characterize the network profile. Furthermore, we confirm the reliability of the system by recapitulating known drug-induced physiological and arrhythmogenic responses. Overall, the model provides a unique cardio-electronic interface for non-invasive measurements of AP dynamics for drug screening and disease modeling.”

Light refreshments will be served @ 1:30pm in EMS E424A.