Osvaldo Sepulveda Villet headshot

Osvaldo Sepulveda Villet

  • Assistant Professor, School Of Freshwater Sciences


I received my B.S. in Aquacultural Engineering from the Universidad Autonoma de Guadalajara (Mexico), and a M.S. in Biology from Central Michigan University. My earlier academic research included nutritional efficiency on freshwater crustaceans, developmental biology of marine shrimp, and proteomics in pharmaceutical discovery. I joined the Great Lakes Genetics Laboratory at the Lake Erie Center, University of Toledo, to focus on yellow perch and percid population genetics and stock assessments for the Great Lakes, particularly Lake Erie and its tributaries. I completed my Ph.D. dissertation, "Population genetic structure and biogeographic patterns in the yellow perch Perca flavescens," in October 2011. I am a recipient of the International Association for Great Lakes Research Scholarship, National Science Foundation GK-12 teaching fellowship, NOAA-Sea Grant John Dean Knauss Fellowship, and USDA ARS HQ Postdoctoral Fellowship. As a recent addition to the School of Freshwater Sciences in the rank of assistant professor, I am focusing on urban and intensive aquaculture of Great Lakes fish species, population genetics of native species in the context of climate change, and the development of novel therapeutic and probiotic agents to reduce the incidence of early-life mortality and disease of cultured fishes.


  • PhD, Biology (Ecology Track), University of Toledo, 2011
  • MS, Biology, Central Michigan University, 2002
  • BS, Aquaculture Engineering, Universidad Autonoma de Guadalajara, Mexico, 1999

Research Interests

  • 13 years of combined Molecular Ecology and genetics research experience, including mitochondrial and nuclear DNA, and RNA analyses. Proficient in theory and technical aspects of population genetics.
  • Experienced in theory and design of Ecology and wildlife management analyses, comprehensive
    knowledge of Great Lakes biotic and abiotic issues and challenges. Direct, hands-on experience in
    reviewing and monitoring federally-funded Great Lakes Restoration Initiative (GLRI) projects.
  • 6 years of experience in maintaining and operating a Molecular Ecology laboratory. Proficient in training and mentoring laboratory personnel.
  • Extensive publication record and demonstrated ability to coordinate and create collaborative research products across academic, state, and federal institutions and agencies.
  • Experienced in theories and practices of aquaculture techniques. Designed and conducted research on nutrition, physiology, and reproductive performance in economically- and ecologically-important species.

Recent and Selected Publications

Sepulveda-Villet, O.J., Shepherd, B.S., Palmquist, D.E. and Binkowski, F.P. In progress. Influence of mineral supplementation using soy-based diets on sex-specific growth and performance in yellow perch. Aquaculture Nutrition.

Sepulveda-Villet, O.J., Shepherd, B.S., Binkowski, F.P., and Sealey, W. 2013. Live feed enrichment with probiotics: perspectives on increasing finfish larviculture success. Global Aquaculture Advocate, July/ August 2013: 71-74.

Shepherd, B.S., Rees, C.B., Sepulveda-Villet, O.J., Palmquist, D.E. and Binkowski, F.P. 2013. Identification of Gender in Yellow Perch by External Morphology: Validation in four Geographic Strains and Effects of Dietary Estradiol. North American Journal of Aquaculture 75: 361-372.

Sepulveda-Villet, O.J., and Stepien, C.A. 2012. Waterscape genetics of the yellow perch (Perca flavescens): patterns across large connected ecosystems and isolated relict populations. Molecular Ecology 21: 5795–5826.

Sepulveda-Villet, O.J., and Stepien, C.A. 2011. Fine-scale population genetic structure of the yellow perch Perca flavescens in Lake Erie. Canadian Journal of Fisheries and Aquatic Sciences 68: 1435-1453.