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Steven Forst

Professor
 (414) 229-6373
 Lapham Hall 458

Education

  • PhD, New York University Medical Center, 1985
  • MS, Rutgers University, 1976
  • BS, Wilkes University, 1974

Research Interests

Our laboratory is interested in understanding the mechanisms by which bacteria adapt to different host environments. We study as a model system Xenorhabdus nematophila, a motile gram-negative bacterium that engages in both mutualistic and pathogenic host interactions. Xenorhabdus forms a species-specific mutualistic association with the insect-invading nematode, Steinernema carpocapsae. The nematode enters insect hosts and X. nematophila is released from an intestinal vesicle into the insect’s body cavity (hemocoel) where it functions as a pathogen. Contraction of the esophagus of the nematode stimulates the forward movement of Xenorhabdus out of the vesicle through a connecting structure that allows the bacteria to enter the intestine and ultimately leave the nematode via the anus. In the hemocoel, Xenorhabdus initially colonizes the connective tissue surrounding the anterior midgut of the host insect. The bacteria proliferate in the insect cadaver reaching high cell densities at which point they produce diverse exoenzymes and antibiotics. The bacteria themselves, as well as the macromolecular degradation they stimulate, provide a nutrient base suitable for nematode reproduction. After several cycles of sexual reproduction the nematodes develop into a dauer juvenile stage that possesses the specialized intestinal vesicle that Xenorhabdus colonizes by a monoclonal process.

Selected Publications

Das, Seema, Singh, Swati, Michael, McClelland, Forst, Steven A., and Prasad, Gyaneshwar. “Characterization of an acid-inducible sulfatase in Salmonella typhimurium LT2.” Appl. Environ. Microbiol. 79.6 (2013): 2092-2095.
Dornfeld, C. L., Hoelzer, M., and Forst, Steven A. “Proteopedia entry: beta-prime subunit of bacterial RNA polymerase.” Biochem. Mol. Biol. Educ. 40.4 (2012): 284.
Morales-Soto, N., Gaudriault, S., Ogier, J. C., Thappeta, K. R., and Forst, Steven A. “Comparative analysis of P2-type remnant prophage loci in Xenorhabdus bovienii and Xenorhabdus nematophila required for xenorhabdicin production.” FEMS Microbiol Lett. 333.1 (2012): 69-76.
Morales-Soto, N., and Forst, Steven A. “The xnp1 P2-like tail synthesis gene cluster encodes xenorhabdicin and is required for interspecies competition.” Journal of Bacteriology 193.14 (2011): 3624-32.
Snyder, H., He, H., Owen, H., Hanna, C., and Forst, Steven A. “Role of Mrx fimbriae of Xenorhabdus nematophila in competitive colonization of the nematode host.” Appl. Environ. Microbiol. 77.20 (2011): 7247-7254.
Ogier, J. C., Calteau, A., Forst, Steven A., Goodrich-Blair, H., Roche, D., Rouy, Z., Zuen, G., Zumbihl, R., Givaudan, A., Tailliez, P., Médigue, C., and Gaudriault, S. “Units of plasticity in bacterial genomes: new insight from the comparative genomics of two bacteria interacting with invertebrates, Photorhabdus and Xenorhabdus.” BMC Genomics 11. (2010): 568-578.
Fodor, A., Fodor, A. M., Forst, Steven A., Hogan, J. S., Klein, M., Lengyel, K., Saringer, G., Stackebrandt, E., TAylor, J., and Lehoczky, E. “Comparative analysis of antibacterial activities of Xenorhabdus species on related and non-related bacteria in vivo.” Journal Microbiol and Antimicrobials 2.3 (2010): 30-35.